“Expression of keratinase enzyme isolated from native bacillus strains in Escherichia coli expression system” is the title of the research project of Amir Maimandipour, a member of the National Research Institute of Genetic Engineering and Biotechnology, which was completed with the support of the National Science Foundation of Iran.
According to Isna“Expression of keratinase enzyme isolated from native Bacillus strains in Escherichia coli expression system” is the title of the research project of Amir Maimandipour, a member of the faculty of the National Institute of Genetic Engineering and Biotechnology, which was completed with the support of the National Science Foundation of Iran.
Amir Maimandipour explained about this plan: Today, slaughterhouse waste is used as a nutritional source with low nutritional value in livestock feeding. Feather is one of the most important wastes of the poultry industry, which accounts for about 5% of the total weight of poultry. The expansion of the poultry industry has led to an increase in this waste, which needs to be managed and transformed into products with more added value.
He added: Creatine forms a major part of feather protein. There are many disulfide bonds in the structure of creatine, which makes this protein insoluble. Keratinases have been previously isolated from microorganisms such as fungi, some bacteria, and Streptomyces. These enzymes have found many uses not only in sewage systems, but in recent years in industries such as food, clothing, medicine and cosmetics.
He continued: Considering that the animal husbandry industry is the second largest industry in Iran and considering its extent, the economic use of production waste of this industry is very important. Therefore, this research has been conducted to investigate the production of high protein flour using biological technologies for use in livestock and poultry feed.
This researcher further stated: In this research project, an attempt has been made to isolate the gene encoding the keratinase enzyme from one of the local bacteria producing this enzyme and express it in E.coli bacteria and investigate its enzymatic properties.
Quoting Iran’s National Science Foundation (INSF), he stated: Using the technical knowledge that has been created and the developed bacterial strain, it is possible to prepare feather flour. The strains obtained from this research will be used to produce the enzyme directly or by adding it to the feathers separated in the slaughterhouse to produce feather meal.
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